3.1.2 Carbohydrates

AQA A-level Biology 3.1.2 practice on Carbohydrates, with free MCQs, clues and worked explanations drawn from the Biological molecules section of specification 7402.

Specification route
3.1.2
Question bank
30 questions
Course stage
AS / Year 12

Sample questions

QUESTION 1 · 3.1.2 · LEVEL 4

A student carries out a Benedict's test on a sample of sucrose. What steps must be taken to produce a positive result?

  • Boil the sample with dilute hydrochloric acid, neutralise with an alkali, then heat with Benedict's solution.
  • Heat the sample with Benedict's solution until it boils.
  • Add dilute sodium hydroxide to the sample, then add iodine solution.
  • Boil the sample with water, cool, then add Benedict's solution at room temperature.
Show clue

Sucrose is a non-reducing sugar, so its glycosidic bond must be broken before the test can work.

Show answer and explanation

Answer: Boil the sample with dilute hydrochloric acid, neutralise with an alkali, then heat with Benedict's solution.

Sucrose is a non-reducing sugar. It must first be hydrolysed into its constituent monosaccharides (glucose and fructose) by boiling with acid, then neutralised, before testing with Benedict's solution to yield a positive result.

QUESTION 2 · 3.1.2 · LEVEL 4

How does the structure of cellulose relate to its function in plant cell walls?

  • It is composed of long, unbranched chains of $\beta$-glucose linked by hydrogen bonds to form strong microfibrils.
  • It is highly branched, allowing for rapid hydrolysis to release $\beta$-glucose for respiration.
  • It is coiled into a compact helical structure, providing dense energy storage in the cell wall.
  • It is made of $\alpha$-glucose chains that fold into a rigid tertiary structure, resisting osmotic pressure.
Show clue

Plant cell walls require high tensile strength rather than energy storage capability.

Show answer and explanation

Answer: It is composed of long, unbranched chains of $\beta$-glucose linked by hydrogen bonds to form strong microfibrils.

Cellulose is formed by the condensation of $\beta$-glucose. These form long, unbranched chains that run parallel to each other, allowing hydrogen bonds to form cross-linkages between chains, creating strong microfibrils.

QUESTION 3 · 3.1.2 · LEVEL 3

Which feature makes glycogen an efficient storage molecule in animal cells?

  • It is highly branched, allowing for rapid hydrolysis to release glucose for respiration.
  • It is a long, unbranched chain that prevents osmotic swelling.
  • It consists of $\beta$-glucose molecules that are highly resistant to enzymatic breakdown.
  • It forms strong microfibrils that can be stored outside the cell-surface membrane.
Show clue

Animals often require rapid bursts of energy.

Show answer and explanation

Answer: It is highly branched, allowing for rapid hydrolysis to release glucose for respiration.

Glycogen is formed by the condensation of $\alpha$-glucose. Its highly branched structure means it has more ends that can be acted on simultaneously by enzymes, allowing rapid hydrolysis into glucose.

QUESTION 4 · 3.1.2 · LEVEL 3

A student tests a solution with iodine in potassium iodide, and it remains yellow-brown. They then heat the solution with Benedict's reagent, and a brick-red precipitate forms. What can be concluded about the solution?

  • It contains a reducing sugar but no starch.
  • It contains a non-reducing sugar but no starch.
  • It contains starch and a reducing sugar.
  • It contains a protein and a non-reducing sugar.
Show clue

Iodine tests for starch; Benedict's (without prior acid hydrolysis) tests for a specific type of sugar.

Show answer and explanation

Answer: It contains a reducing sugar but no starch.

A negative iodine test (remains yellow-brown) indicates no starch. A positive Benedict's test (brick-red precipitate upon heating) indicates the presence of reducing sugars.

QUESTION 5 · 3.1.2 · LEVEL 3

Glucose is described as a hexose sugar and has the formula $C_{6}H_{12}O_{6}$. What makes $\alpha$-glucose an isomer of $\beta$-glucose?

  • They have the same molecular formula but a different structural arrangement of atoms.
  • They have different molecular formulas but belong to the same functional group.
  • They have the same structural arrangement but a different number of carbon atoms.
  • They form different types of glycosidic bonds but yield the same polymers.
Show clue

The definition of an isomer relates to the formula and structural arrangement.

Show answer and explanation

Answer: They have the same molecular formula but a different structural arrangement of atoms.

Glucose has two isomers, alpha-glucose and beta-glucose. Isomers are molecules that have the same molecular formula ($C_{6}H_{12}O_{6}$) but a different structural arrangement of atoms in space (the position of the hydroxyl group on carbon 1).

QUESTION 6 · 3.1.2 · LEVEL 1

Which two monosaccharides condense to form a molecule of sucrose?

  • Glucose and fructose
  • Glucose and glucose
  • Glucose and galactose
  • Galactose and fructose
Show clue

Sucrose is common table sugar, produced heavily by plants.

Show answer and explanation

Answer: Glucose and fructose

Sucrose is a disaccharide formed by condensation of a glucose molecule and a fructose molecule.

QUESTION 7 · 3.1.2 · LEVEL 1

Which of the following disaccharides is formed by the condensation of two glucose molecules?

  • Maltose
  • Sucrose
  • Lactose
  • Cellobiose
Show clue

This sugar is often found in germinating seeds and malt.

Show answer and explanation

Answer: Maltose

Maltose is a disaccharide formed by condensation of two glucose molecules.

QUESTION 8 · 3.1.2 · LEVEL 2

Which polymer is formed by the condensation of $\beta$-glucose units?

  • Cellulose
  • Glycogen
  • Starch
  • Amylopectin
Show clue

This polymer provides structural support in plant cell walls.

Show answer and explanation

Answer: Cellulose

Cellulose is formed by the condensation of beta-glucose. Glycogen and starch are formed by the condensation of alpha-glucose.

Practise this topic

30 questions are available. No sign-in required.